Separation of acid and neutral cannabinoids in Cannabis sativa L. using HPLC
Publication 17
Biology of Cannabinoids, 79-88
Jocelyn C. Turner & Paul G. Mahlberg
Department of Biology, Indiana University, Bloomington, IN 47405
Keywords: Cannabis sativa, Cannabaceae, cannabinoids, HPLC, acid, neutral
Abstract
Chemobotanical studies in our laboratory on the site of cannabinoid. synthesis in the Cannabis plant required a method for an accurate assessment of the cannabinoid profile. Previous methods primarily involved use of gas-liquid chromatography (GLC) (1,2). Because the majority of cannabinoids are present in the plant are found in the acid form, they cannot be directly detected by GLC due to thermal decarboxylation. High Performance Liquid Chromatography (HPLC) is able to detect. directly both acidic and neutral cannabinoids. However, methods published to date were found to be inadequate for our use (3,4). ‘The abundance of compounds in samples extracted from plant material made their analyses complex, and necessitated development of a more definitive method for determining cannabinoid profiles in plant samples.
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