Plastid development in glandular trichomes of Cannabis (Cannabaceae)
Publication 31
J. Plant Biol. 40(1) : 61-66 (1997)
Eun Soo Kim & Paul G. Mahlberg
Department of Biology, Konkuk University, Seoul 143-701, Korea;
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Keywords: Cannabis, glands, celluase, cytochemistry, secretory cavity
Abstract
Plastids in lipophilic glandular trichomes of chemically fixed (CF) and high pressure cryofixed- cryosubstituted (UPC-CS) bracteal tissues of Cannabis were examined by transmission electron microscopy. In CF preparations, plastids in disc cells prior to secretory cavity formation possessed several lobed and dilated thylakoid-like features. In glands with secretory cavities, thylakoid-like features aggregated to form reticulate bodies that distended regions of the elongated plastids. Electron-gray inclusions evident on the plastid surface appeared continuous with the reticulate body. Inclusions of similar electron density also appeared in the cell cytoplasm, along the plasma membrane, between the plasma membrane and cell wall facing the cavity, and in the secretory cavity in both CF and UPC-CS preparations. The bilayer structure of membranes of the plastid envelope was evident in UPC-CS but not in CF preparations. In UPC-CS preparations, secretions were evident on the plastid surface and were continuous with those in the plastid through pores in the envelope. This study supports an interpretation that these specialized plastids, lipoplasts, synthesize secretions that are transported through the plasma membrane and cell wall to subsequently accumulate in the secretory cavity.
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