Journal Of Natural Products, Vol. 44, No. 2, Mar-Apr, 1981
Marion Furr & Paul G. Mahlberg
Department of Biology, Indiana University, Bloomington, IN 47405
Keywords: Cannabis sativa, Cannabaceae, cannabinoids, Histochemical, Laticifers, Analyses
The unbranched nonarticulated laticifer, including its latex, and capitate glandular trichomes from Cannabis saliva L. were analyzed in fresh and cryostat preparations with histochemical procedures for the presence of cannabinoids, alkalis, and other selected cellular components. A positive response to cannabinoid indicators, Duquenois-Negm, fast blue salt B, Gibb, and Beam reagents occurred in laticifers, as well as exuded latex and in disc cells of epidermal capitate glandular trichomes. No response or only an apparent background response to these reagents was detected in other cells. Alkaloids were detected histochemically in laticifers and exuded latex with Wagner, Dittmar, Ellram, chromic acid, Hager, and Dragendorff reagents. Alkaloid indicators also reacted with capitate glandular trichomes but did not show a positive response in other cells of the plant. Laticifers also contained other specialized contents including the enzymes, cytochrome oxidase, and lipase. Free lipids and storage proteins were not detected in laticifers. Qualitative responses to these histochemical procedures were similar in laticifers and capitate glandular trichomes from various regions and organs of the plant axis. Histochemical indicators can be useful for preliminary surveying of specialized cell types and tissues for the presence of specialized substances.
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