Effects of sample treatment on chromatographic analysis of cannabinoids in Cannabis sativa L. (Cannabaceae)
Publication 18
Journal of Chromatography, 283 (1984) 165-171
Jocelyn C. Turner & Paul G. Mahlberg
Department of Biology, Indiana University, Bloomington, IN 47405
(Received August 31st, 1983)
Keywords: Cannabis sativa, Cannabaceae, cannabinoids, chromatography, analysis
Abstract
Gas-liquid chromatographic and high-performance liquid chromatographic analyses on the effects of leaf treatment as well as the conditions for cannabinoid extraction were examined in two clones of Cannabis sativa L. Cannabinoid extracts of dried leaves, when analyzed by gas-liquid chromatography, showed no significant quantitative or qualitative differences regardless of drying procedure or temperature and duration of extraction investigated. Comparable high-performance liquid chromatographic analyses, however, indicated that while extraction temperature did not influence the cannabinoid profile, drying conditions had a significant effect. High ratios of acid to neutral forms were derived only from extracts of leaves dried at 37°C as compared to 60°C. Fresh, non-dried leaf material also yielded high ratios of acid to neutral forms, but the duration of extraction was found to affect cannabinoid yield significantly. Longer extractions of fresh leaves resulted in lower amounts of cannabinoids extracted. This study determined optimal procedures for analyzing fresh plant materials.
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